Anti alfa-CGRP (calcitonin gene-related peptide, human)
Mouse Monoclonal Antibody
Cat.No. ABS 026-05
Preparation: Protein-A/G purified
Content: Available in 200 µL and 1 mL, 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15mM sodium azide
Storage: In the dark at 4-8ºC
Calcitonin gene-related peptide is a 37 amino-acid vasoactive neuropeptide (mw 3789) widely expressed throughout the central and peripheral nervous system. CGRP has potent vasodilatory effects probably serving as a regulator of regional blood flow, in addition to a variety of other cellular effects.
Synthetic human alfa-CGRP coupled to carrier
ABS 026-05 binds human alfa-CGRP. Based on inhibition ELISA, ABS 026-05 shows full cross-reactivity with rat alfa-CGRP and about about 50% cross-reactivity with human beta-CGRP. Cross-reactivity with coated rat alfa-CGRP is low (8%) and there is no cross-reaction with human amylin.
The epitope lies within residues 1-18 of human alfa-CGRP
ABS 026-05 binds free alfa-CGRP in solution. Based on inhibition ELISA, the affinity of ABS 026-05 is at least 24 times higher than that of ABS 026-04.
The ELISA dilution guideline of applies to human alfa-CGRP coated directly at 1.0 g/mL. showing that the antibody binds poorly to the denatured peptide.
ABS 026-05 (as capture antibody) forms a sandwich ELISA pair with ABS 026-04 (as biotinylated detection antibody) for measuring human alfa-CGRP, giving a detection limit of 0.13 nmol/L in an unoptimized buffer assay.
| Method | Usability | Dilution guideline | References |
| ELISA | Yes | 1:2000 | |
| Immunoblotting | Not determined | ||
| Immunohistochemistry | Not determined |
